pgl3 basic 180 luciferase reporter plasmid (Addgene inc)
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Pgl3 Basic 180 Luciferase Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+basic+luciferase+reporter+plasmid/pGL3+(-BRE)+Luciferase+(Plasmid+%2345127)/10__1158_slash_0008___5472__can___25___2893-63-26-31
Average 95 stars, based on 195 article reviews
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Plasmid Preparation:Article Title: Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation Article Snippet: .. The pGL3-ARE-luc vector was generated by inserting three copies of the antioxidant response element (ARE; 5'-GTGACAAAGCAATCCCGT GACAAAGCAATCCCGTGACAAAGCAATA-3') into Article Title: PPARγ induces NEDD4 gene expression to promote autophagy and insulin action. Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/febs.15042 This article is protected by copyright.. All rights reserved. Article Title: MicroRNA‑218 inhibits the malignant phenotypes of glioma by modulating the TNC/AKT/AP‑1/TGFβ1 feedback signaling loop. Article Snippet: .. The 3xAP in Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Article Title: YAP induces an oncogenic transcriptional program through TET1-mediated epigenetic remodeling in liver growth and tumorigenesis. Article Snippet: 1Department of Physiology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA.. 2Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.. ✉e-mail: duojia.pan@utsouthwestern.edu The preexisting epigenetic landscape represents a barrier to oncogene-induced cellular transformation and malignancy. Article Title: MiT/ TFE factors control ER ‐phagy via transcriptional regulation of FAM 134B Article Snippet: .. The promoter region of rat Fam134b from 78211858 to 78211187 (CLEAR 108404, −2708 from TSS position in intron 3) was amplified by PCR from RCS chondrocyte genome and cloned into Generated:Article Title: Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation Article Snippet: .. The pGL3-ARE-luc vector was generated by inserting three copies of the antioxidant response element (ARE; 5'-GTGACAAAGCAATCCCGT GACAAAGCAATCCCGTGACAAAGCAATA-3') into Luciferase:Article Title: Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation Article Snippet: .. The pGL3-ARE-luc vector was generated by inserting three copies of the antioxidant response element (ARE; 5'-GTGACAAAGCAATCCCGT GACAAAGCAATCCCGTGACAAAGCAATA-3') into Article Title: PPARγ induces NEDD4 gene expression to promote autophagy and insulin action. Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/febs.15042 This article is protected by copyright.. All rights reserved. Article Title: MicroRNA‑218 inhibits the malignant phenotypes of glioma by modulating the TNC/AKT/AP‑1/TGFβ1 feedback signaling loop. Article Snippet: .. The 3xAP in Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Article Title: YAP induces an oncogenic transcriptional program through TET1-mediated epigenetic remodeling in liver growth and tumorigenesis. Article Snippet: 1Department of Physiology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA.. 2Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.. ✉e-mail: duojia.pan@utsouthwestern.edu The preexisting epigenetic landscape represents a barrier to oncogene-induced cellular transformation and malignancy. Article Title: MiT/ TFE factors control ER ‐phagy via transcriptional regulation of FAM 134B Article Snippet: .. The promoter region of rat Fam134b from 78211858 to 78211187 (CLEAR 108404, −2708 from TSS position in intron 3) was amplified by PCR from RCS chondrocyte genome and cloned into Construct:Article Title: Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation Article Snippet: .. The pGL3-ARE-luc vector was generated by inserting three copies of the antioxidant response element (ARE; 5'-GTGACAAAGCAATCCCGT GACAAAGCAATCCCGTGACAAAGCAATA-3') into Article Title: PPARγ induces NEDD4 gene expression to promote autophagy and insulin action. Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/febs.15042 This article is protected by copyright.. All rights reserved. shRNA:Article Title: Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation Article Snippet: .. The pGL3-ARE-luc vector was generated by inserting three copies of the antioxidant response element (ARE; 5'-GTGACAAAGCAATCCCGT GACAAAGCAATCCCGTGACAAAGCAATA-3') into Polymerase Chain Reaction:Article Title: PPARγ induces NEDD4 gene expression to promote autophagy and insulin action. Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/febs.15042 This article is protected by copyright.. All rights reserved. Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Article Title: MiT/ TFE factors control ER ‐phagy via transcriptional regulation of FAM 134B Article Snippet: .. The promoter region of rat Fam134b from 78211858 to 78211187 (CLEAR 108404, −2708 from TSS position in intron 3) was amplified by PCR from RCS chondrocyte genome and cloned into Cloning:Article Title: PPARγ induces NEDD4 gene expression to promote autophagy and insulin action. Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/febs.15042 This article is protected by copyright.. All rights reserved. Binding Assay:Article Title: MicroRNA‑218 inhibits the malignant phenotypes of glioma by modulating the TNC/AKT/AP‑1/TGFβ1 feedback signaling loop. Article Snippet: .. The 3xAP in Amplification:Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Article Title: MiT/ TFE factors control ER ‐phagy via transcriptional regulation of FAM 134B Article Snippet: .. The promoter region of rat Fam134b from 78211858 to 78211187 (CLEAR 108404, −2708 from TSS position in intron 3) was amplified by PCR from RCS chondrocyte genome and cloned into Clone Assay:Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Article Title: MiT/ TFE factors control ER ‐phagy via transcriptional regulation of FAM 134B Article Snippet: .. The promoter region of rat Fam134b from 78211858 to 78211187 (CLEAR 108404, −2708 from TSS position in intron 3) was amplified by PCR from RCS chondrocyte genome and cloned into other:Article Title: MicroRNA-218 inhibits the malignant phenotypes of glioma by modulating the TNC/AKT/AP-1/TGFβ1 feedback signaling loop Article Snippet: The wild-type (WT) TNC 3′-UTR was amplified from cDNA of U251 cells to construct the luciferase reporter plasmids The TNC 3′-UTR with mutant (MUT) binding site of miR-218 was synthesized by Sangon Biotech Co, Ltd These two fragments were inserted into pre-digested pmirGLO luciferase vector (gifted by Dr Yanke Chen at Xi'an Jiaotong University Health Science Center) to produce the luciferase reporter plasmids, pmirGLO-TNC 3′-UTR-WT and pmirGLO-TNC 3′-UTR-MUT The primer sequences for plasmid constructs are listed in The 3×AP in |
